Liquid chromatography (LC) is a type of chromatography that separates non-volatile compounds based on their different interactions with a stationary phase and a mobile phase. In LC, a mixture is dissolved in a liquid, known as the mobile phase, and passed through a column containing a stationary phase, such as a silica gel or a polymer. The components in the mixture interact differently with the stationary phase and mobile phase, resulting in their separation. The components are then detected by a detector, such as a UV detector or a fluorescence detector, and identified based on their unique characteristics.
Ion exchange chromatography (IEC) is a type of Chromatography that separates charged compounds based on their different affinities for a stationary phase and a mobile phase. In IEC, a mixture is passed through a column containing a stationary phase, such as a resin or a gel, that has charged functional groups. The mobile phase is a liquid that contains ions of opposite charge to the stationary phase. The components in the mixture interact differently with the stationary phase and mobile phase, resulting in their separation. The components are then detected by a detector, such as a conductivity detector or a UV detector, and identified based on their unique characteristics.
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